Interaction Details


Details of experiments between O43426 and P11021
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: HSPA5
Fragment: 1-654
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.33
Immobilized partner concentration (10-6M): 10
P value: 11.96
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-12-11
Number of measurements: 6
Measured pKd: 4.7
Experimental Details

Batch = Helsinki_batch5; Ncontrols = 12; Nsamples = 6; Npreys = 6006; threshold curvature: 1.54

Experiment series 2

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: HSPA5
Fragment: 1-654
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.29
Immobilized partner concentration (10-6M): 10
P value: 12.31
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
Measured pKd: 4.62
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.72