Interaction Details


Details of experiments between O43379 and P62258
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: WDR62
Fragment: 1223-1232
Site: 14-3-3_pSer1228
Modification: (SEP)1228
Sequence: ISRSISLGDS
Construct: ISRSI(pSer)LGDS (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.49
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 116
P value: 6.45
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.93
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: WDR62
Fragment: 1223-1232
Site: 14-3-3_pSer1228
Modification: (SEP)1228
Sequence: ISRSISLGDS
Construct: ISRSI(pSer)LGDS (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.5
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 143
P value: 7.56
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.85
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: WDR62
Fragment: 1244-1253
Site: 14-3-3_pSer1249
Modification: (SEP)1249
Sequence: LRRPSSVGEL
Construct: LRRPS(pSer)VGEL (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.26
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 143
P value: 3.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.39
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: WDR62
Fragment: 1244-1253
Site: 14-3-3_pSer1249
Modification: (SEP)1249
Sequence: LRRPSSVGEL
Construct: LRRPS(pSer)VGEL (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.17
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 116
P value: 2.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: WDR62
Fragment: 1337-1346
Site: 14-3-3_pSer1342
Modification: (SEP)1342
Sequence: SAFRPSLPAP
Construct: SAFRP(pSer)LPAP (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.03
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 116
P value: 0.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: WDR62
Fragment: 1337-1346
Site: 14-3-3_pSer1342
Modification: (SEP)1342
Sequence: SAFRPSLPAP
Construct: SAFRP(pSer)LPAP (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.02
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 143
P value: 0.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: WDR62
Fragment: 892-901
Site: 14-3-3_pSer897
Modification: (SEP)897
Sequence: ESLENSILDS
Construct: ESLEN(pSer)ILDS (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.24
Holdup BI standard deviation: 0.35
Immobilized partner concentration (10-6M): 116
P value: 0.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: WDR62
Fragment: 892-901
Site: 14-3-3_pSer897
Modification: (SEP)897
Sequence: ESLENSILDS
Construct: ESLEN(pSer)ILDS (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: -0.13
Holdup BI standard deviation: 0.38
Immobilized partner concentration (10-6M): 143
P value: 0.33
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))