Interaction Details


Details of experiments between O43182 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGAP6
Fragment: 965-974
Site: PBM
Sequence: NPDALPETLV
Construct: biotin-ttds-NPDALPETLV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.46
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.46
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 4.7
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ARHGAP6
Fragment: 965-974
Site: PBM
Sequence: NPDALPETLV
Construct: biotin-ttds-NPDALPETLV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 21.05
Standard deviation of dissociation constant (10-6M): 0.88
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.68
PUBMED ID: 36115835