Interaction Details


Details of experiments between O43182 and Q68DX3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGAP6
Fragment: 965-974
Site: PBM
Sequence: NPDALPETLV
Construct: biotin-ttds-NPDALPETLV

Protein B

Protein: FRMPD2
Fragment: 944-1038
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.55
Normalized holdup BI: 0.61
Immobilized partner concentration (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.99
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ARHGAP6
Fragment: 965-974
Site: PBM
Sequence: NPDALPETLV
Construct: biotin-ttds-NPDALPETLV

Protein B

Protein: FRMPD2
Fragment: 1073-1166
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: ARHGAP6
Fragment: 965-974
Site: PBM
Sequence: NPDALPETLV
Construct: biotin-ttds-NPDALPETLV

Protein B

Protein: FRMPD2
Fragment: 770-861
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835