Interaction Details


Details of experiments between O43166 and P06463
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SIPA1L1
Fragment: 946-1030
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Average holdup BI: -0.04
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 13.3
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835