Interaction Details


Details of experiments between O15446 and NC_000001.11
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: POLR1G
Fragment: 1-510
Construct: endogenous protein (K562)

Protein B

Protein: 51_HAP
Fragment: 203681832-203681862
Construct: Biotin-GAATTGAGAGGTATCTTACCGCTCCCACTCC
Average holdup BI: 0.69
Immobilized partner concentration (10-6M): 5
P value: 7.08
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.64
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: POLR1G
Fragment: 1-510
Construct: endogenous protein (K562)

Protein B

Protein: 4945_HAP
Fragment: 203681644-203681669
Construct: Biotin-TCAGCCCTCCGTTTTGTCACCTACAC
Average holdup BI: 0.58
Immobilized partner concentration (10-6M): 5
P value: 7.39
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.43
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 3

Protein A

Protein: POLR1G
Fragment: 1-510
Construct: endogenous protein (K562)

Protein B

Protein: 4945_WT
Fragment: 203681644-203681669
Modification: rs10751449/rs10736845
Construct: Biotin-TCAGCCCTCCGTATCGTCACCTACAC
Average holdup BI: 0.64
Immobilized partner concentration (10-6M): 5
P value: 8.03
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.54
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 4

Protein A

Protein: POLR1G
Fragment: 1-510
Construct: endogenous protein (K562)

Protein B

Protein: 51_WT
Fragment: 203681832-203681862
Modification: rs10751451
Construct: Biotin-GAATTGAGAGGTATCTTATCGCTCCCACTCC
Average holdup BI: 0.7
Immobilized partner concentration (10-6M): 5
P value: 7.65
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.67
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))