Interaction Details


Details of experiments between O15439 and Q6P0Q8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.92
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.92
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.92
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.9
Normalized holdup BI: 0.9
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.8
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 3

Protein A

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.81
Normalized holdup BI: 0.9
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.77
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 4

Protein A

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: Biotin-ado-ado-STLTIFETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.87
Normalized holdup BI: 0.87
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.67
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.6
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.3
PUBMED ID: 36115835