Interaction Details


Details of experiments between O15439 and O14907
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: Biotin-ado-ado-STLTIFETAL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.69
Normalized holdup BI: 0.69
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.16
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.6
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.66
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 5.11
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.55
Normalized holdup BI: 0.61
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 4

Protein A

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.43
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.96
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: ABCC4
Fragment: 1-1325
Construct: endogenous protein (Jurkat)

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.45
Immobilized partner concentration (10-6M): 10
P value: 2.55
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))