Interaction Details


Details of experiments between O15169 and Q9P202
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AXIN1
Fragment: 333-347
Site: Internal_PBM
Construct: Biotin-GSGSGS-TLSLTDSSVDGIPPYR-CONH2

Protein B

Protein: DFNB31
Fragment: 813-904
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.91
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: AXIN1
Fragment: 333-347
Site: Internal_PBM
Construct: Biotin-GSGSGS-TLSLTDSSVDGIPPYR-CONH2

Protein B

Protein: DFNB31
Fragment: 136-226
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: AXIN1
Fragment: 333-347
Site: Internal_PBM
Construct: Biotin-GSGSGS-TLSLTDSSVDGIPPYR-CONH2

Protein B

Protein: DFNB31
Fragment: 263-379
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835