Interaction Details


Details of experiments between O15169 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AXIN1
Fragment: 333-347
Site: Internal_PBM
Construct: Biotin-GSGSGS-TLSLTDSSVDGIPPYR-CONH2

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.67
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: AXIN1
Fragment: 333-347
Site: Internal_PBM
Construct: Biotin-GSGSGS-TLSLTDSSVDGIPPYR-CONH2

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835