Interaction Details


Details of experiments between O15085 and Q15418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 726-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: Biotin-ado-ado-RVRKLPSTTL
Average holdup BI: 0.27
Normalized holdup BI: 0.46
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.72
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL
Average holdup BI: 0.25
Normalized holdup BI: 0.32
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.44
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL
Average holdup BI: 0.44
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 31
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
Measured pKd: 4.44
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.06
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.77
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.13
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 40
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 4
Measured pKd: 3.57
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)733
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSpTTL
Average holdup BI: -0
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (TPO)734
Sequence: RVRKLPSTTL
Construct: Biotin-ttds-RRVRKLPSTpTL
Average holdup BI: 0.24
Normalized holdup BI: 0.32
Immobilized partner concentration (10-6M): 25
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.29
PUBMED ID: 36115835