Interaction Details

Details of experiments between O15085 and O43426
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: ARHGEF11
Fragment: 1-1522
Construct: endogenous proteins (Jurkat)

Protein B

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Average holdup BI: -0.03

Immobilized partner concentration (10^-6M): 10

P value: 0.12

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic equation

Date of measurement: 2023-12-11

Number of measurements: 6

The affinity was below the detection threshold of the assay.

Experimental Details

Batch = Helsinki_batch5; Ncontrols = 12; Nsamples = 6; Npreys = 6006; threshold curvature: 1.54