Interaction Details


Details of experiments between O15056 and Q14155
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ2
Fragment: 1-1496
Construct: endogenous protein (Jurkat)

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.77
Immobilized partner concentration (10-6M): 10
P value: 4.44
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.52
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: SYNJ2
Fragment: 1219-1233
Site: PxxP_5
Sequence: ETPQAPPLLPRRPPP
Construct: Bio Ado Ado-ETPQAPPLLPRRPPP-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.97
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: SYNJ2
Fragment: 1324-1338
Site: PxxP_7
Sequence: EAPPLVPKVPPRRKK
Construct: Bio Ado Ado-EAPPLVPKVPPRRKK-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.58
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.94
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: SYNJ2
Fragment: 1227-1241
Site: PxxP_6
Sequence: LPRRPPPRVPAIKKP
Construct: Bio Ado Ado-LPRRPPPRVPAIKKP-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.36
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: SYNJ2
Fragment: 1098-1112
Site: PxxP_1
Sequence: VPNRPRPPQPPQRPP
Construct: Bio Ado Ado-VPNRPRPPQPPQRPP-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.78
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: SYNJ2
Fragment: 1099-1113
Site: PxxP_2
Sequence: PNRPRPPQPPQRPPP
Construct: Bio Ado Ado-PNRPRPPQPPQRPPP-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.63
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: SYNJ2
Fragment: 1140-1154
Site: PxxP_3
Sequence: QTARLLPGAPQQPPK
Construct: Bio Ado Ado-QTARLLPGAPQQPPK-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: SYNJ2
Fragment: 1143-1157
Site: PxxP_4
Sequence: RLLPGAPQQPPKART
Construct: Bio Ado Ado-RLLPGAPQQPPKART-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: SYNJ2
Fragment: 1442-1456
Site: PxxP_8
Sequence: RSPKRDPIDPVSAGA
Construct: Bio Ado Ado-RSPKRDPIDPVSAGA-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680