Interaction Details

Details of experiments between O15021 and Q9UHC3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: MAST4
Fragment: 1140-1232
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Average holdup BI: 0

Immobilized partner concentration (10^-6M): 11.5

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.