Interaction Details


Details of experiments between O15021 and P06463
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST4
Fragment: 1140-1232
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 13.3
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835