Interaction Details


Details of experiments between O15021 and O75084
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST4
Fragment: 1140-1232
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835