Interaction Details


Details of experiments between O15018 and Q02643
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GHRHR
Fragment: 414-423
Site: PBM
Sequence: SAAKVLTSMC
Construct: biotin-ado-ado-SAAKVLTSMC
Average holdup BI: 0.47
Normalized holdup BI: 0.47
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.75
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.