Interaction Details

Details of experiments between O15018 and P60484
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PDZD2
Fragment: 2611-2718
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Average holdup BI: 0.2

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

Measured pK_d: 4.17

PUBMED ID: 33382810

Experiment series 2

Protein A

Protein: PDZD2
Fragment: 722-815
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Average holdup BI: 0.11

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

Measured pK_d: 3.86

PUBMED ID: 33382810

Experiment series 3

Protein A

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Average holdup BI: 0.09

Normalized holdup BI: 0.09

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.71

PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Average holdup BI: 0.06

Normalized holdup BI: 0.06

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.58

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: PDZD2
Fragment: 2741-2839
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Average holdup BI: 0.12

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

Measured pK_d: 3.56

PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: PDZD2
Fragment: 2611-2718
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Average holdup BI: 0.03

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: PDZD2
Fragment: 2741-2839
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Average holdup BI: -0.07

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 8

Protein A

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Average holdup BI: 0.05

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 9

Protein A

Protein: PDZD2
Fragment: 575-684
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Average holdup BI: -0.05

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 10

Protein A

Protein: PDZD2
Fragment: 575-684
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Average holdup BI: -0.07

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: PDZD2
Fragment: 64-181
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Average holdup BI: -0.01

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 12

Protein A

Protein: PDZD2
Fragment: 64-181
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Average holdup BI: -0.01

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 13

Protein A

Protein: PDZD2
Fragment: 722-815
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Average holdup BI: -0.02

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 14

Protein A

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Average holdup BI: 0.28

Normalized holdup BI: 0.28

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.35

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 15

Protein A

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Average holdup BI: 0.26

Normalized holdup BI: 0.26

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.34

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 16

Protein A

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Average holdup BI: 0.21

Normalized holdup BI: 0.21

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.22

PUBMED ID: 36115835

Experiment series 17

Protein A

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Average holdup BI: 0.2

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

Measured pK_d: 3.93

PUBMED ID: 36115835

Experiment series 18

Protein A

Protein: PDZD2
Fragment: 2611-2718
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Average holdup BI: -0.09

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 19

Protein A

Protein: PDZD2
Fragment: 2741-2839
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Average holdup BI: -0.03

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 20

Protein A

Protein: PDZD2
Fragment: 575-684
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Average holdup BI: 0.02

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 21

Protein A

Protein: PDZD2
Fragment: 64-181
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Average holdup BI: -0.1

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 22

Protein A

Protein: PDZD2
Fragment: 722-815
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Average holdup BI: -0.17

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835