Interaction Details


Details of experiments between O15018 and O95049
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TJP3
Fragment: 910-919
Site: PBM
Sequence: GYDWGPATDL
Construct: Biotin-Ahx-GYDWGPATDL
Average holdup BI: 0.33
Normalized holdup BI: 0.33
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 36115835