Interaction Details

Details of experiments between O14974 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PPP1R12A
Fragment: 1-1030
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.67

Immobilized partner concentration (10^-6M): 10

P value: 2.92

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic binding equation

Date of measurement: 2022-06-29

Number of measurements: 3

Measured pK_d: 5.31

PUBMED ID: 38995680

Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: PPP1R12A
Fragment: 1-1030
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.32

Immobilized partner concentration (10^-6M): 10

P value: 6.19

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic binding equation

Date of measurement: 2022-06-29

Number of measurements: 3

Measured pK_d: 4.67

PUBMED ID: 38995680

Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.21

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.18

PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.21

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.18

PUBMED ID: N

Experiment series 5

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.17

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 4.07

PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.03

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.03

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 9

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.01

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.04

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.01

Holdup BI standard deviation: 0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.01

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.05

Holdup BI standard deviation: 0.06

Immobilized partner concentration (10^-6M): 6

Experiment method: HU (DAPF)

Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 3.96

PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.1

Holdup BI standard deviation: 0.01

Normalized holdup BI: 0.12

Normalized holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-08-04

Number of measurements: 2

Measured pK_d: 3.9

PUBMED ID: N

Experiment series 15

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.02

Holdup BI standard deviation: 0.03

Normalized holdup BI: 0.03

Normalized holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-08-04

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 16

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.04

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 6

Experiment method: HU (DAPF)

Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT

Date of measurement: 2022-10-24

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.24

Holdup BI standard deviation: 0.3

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 4.26

PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.19

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 4.13

PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.11

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-08-04

Number of measurements: 2

Measured pK_d: 3.86

PUBMED ID: N

Experiment series 20

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.03

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-08-04

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 21

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.12

Holdup BI standard deviation: 0.11

Immobilized partner concentration (10^-6M): 6

Experiment method: HU (DAPF)

Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 4.39

PUBMED ID: 38995680

Experiment series 22

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.09

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 6

Experiment method: HU (DAPF)

Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 4.21

PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.09

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-08-04

Number of measurements: 2

Measured pK_d: 3.72

PUBMED ID: N

Experiment series 24

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.01

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-08-04

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 25

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.2

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.15

PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.2

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.15

PUBMED ID: N

Experiment series 27

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.16

Holdup BI standard deviation: 0.21

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 4.05

PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.15

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 4

PUBMED ID: 38995680

Experiment series 29

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.04

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 30

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.04

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 31

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.16

Holdup BI standard deviation: 0.18

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 4.04

PUBMED ID: 38995680

Experiment series 32

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.15

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 3.99

PUBMED ID: 38995680

Experiment series 33

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.1

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-08-04

Number of measurements: 2

Measured pK_d: 3.79

PUBMED ID: N

Experiment series 34

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.02

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-08-04

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 35

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.09

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 9

Experiment method: HU (DAPF)

Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT

Date of measurement: 2022-10-24

Number of measurements: 2

Measured pK_d: 4.03

PUBMED ID: 38995680

Experiment series 36

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.02

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 9

Experiment method: HU (DAPF)

Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT

Date of measurement: 2022-10-24

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 37

Protein A

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: -0.01

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 38

Protein A

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.02

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-10-24

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680