Interaction Details

Details of experiments between O14924 and Q9Q6P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: RGS12
Fragment: 3-121
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Measured dissociation constant (10^-6M):

Standard deviation of dissociation constant (10^-6M): 0

Average holdup BI: -0.2

Normalized holdup BI: 0

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

P value: 0

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835