Interaction Details


Details of experiments between O14924 and P06427
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RGS12
Fragment: 3-121
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.5
PUBMED ID: 36115835