Interaction Details


Details of experiments between O14907 and Q9NYB5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL
Average holdup BI: 0.64
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.71
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 11.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 5.47
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL
Average holdup BI: 0.61
Normalized holdup BI: 0.67
Immobilized partner concentration (10-6M): 11.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.38
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 3

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL
Average holdup BI: 0.45
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.35
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: Biotin-ado-ado-NYWPGKETQL
Average holdup BI: 0.76
Normalized holdup BI: 0.76
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.32
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6