Interaction Details


Details of experiments between O14907 and Q8WUF5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PPP1R13L
Fragment: 819-828
Site: PBM
Sequence: PRVKPQRSKV
Construct: biotin-ttds-PRVKPQRSKV
Average holdup BI: 0.09
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.1
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.82
PUBMED ID: 36115835