Interaction Details


Details of experiments between O14907 and Q15311
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.39
Normalized holdup BI: 0.39
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.59
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.28
Normalized holdup BI: 0.31
Immobilized partner concentration (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.23
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 1-655
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.26
Immobilized partner concentration (10-6M): 10
P value: 1.65
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 4

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835