Interaction Details


Details of experiments between O14907 and P25054
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: APC
Fragment: 2834-2843
Site: PBM
Sequence: HSGSYLVTSV
Construct: Biotin-Ahx-HSGSYLVTSV
Average holdup BI: 0.76
Normalized holdup BI: 0.83
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.53
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: APC
Fragment: 2834-2843
Site: PBM
Sequence: HSGSYLVTSV
Construct: Biotin-Ahx-HSGSYLVTSV
Average holdup BI: 0.82
Normalized holdup BI: 0.82
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.48
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6