Interaction Details

Details of experiments between O14745 and Q5SYB0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: NHERF1
Fragment: 349-358
Site: PBM
Sequence: SKKNELFSNL
Construct: Biotin-Ahx-SKKNELFSNL

Protein B

Protein: FRMPD1
Fragment: 53-142
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04

Normalized holdup BI: -0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835