Interaction Details


Details of experiments between O14745 and Q14CM0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF1
Fragment: 10-98
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NHERF1
Fragment: 146-237
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: NHERF1
Fragment: 349-358
Site: PBM
Sequence: SKKNELFSNL
Construct: Biotin-Ahx-SKKNELFSNL

Protein B

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835