Interaction Details


Details of experiments between O14745 and Q02410
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF1
Fragment: 349-358
Site: PBM
Sequence: SKKNELFSNL
Construct: Biotin-Ahx-SKKNELFSNL

Protein B

Protein: APBA1
Fragment: 743-822
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.48
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NHERF1
Fragment: 349-358
Site: PBM
Sequence: SKKNELFSNL
Construct: Biotin-Ahx-SKKNELFSNL

Protein B

Protein: APBA1
Fragment: 655-741
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835