Interaction Details


Details of experiments between O14745 and P60484
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF1
Fragment: 10-98
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 36
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NHERF1
Fragment: 146-237
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
Number of measurements: 1
Measured pKd: 3.97
PUBMED ID: 33382810

Experiment series 3

Protein A

Protein: NHERF1
Fragment: 146-237
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 36
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: NHERF1
Fragment: 10-98
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 29
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.85
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: NHERF1
Fragment: 146-237
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 29
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835