Interaction Details


Details of experiments between O14745 and P23634
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF1
Fragment: 10-98
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B4
Fragment: 1232-1241
Site: PBM
Sequence: SSLQSLETSV
Construct: biotin-ttds-SSLQSLETSV
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NHERF1
Fragment: 146-237
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B4
Fragment: 1232-1241
Site: PBM
Sequence: SSLQSLETSV
Construct: biotin-ttds-SSLQSLETSV
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835