Interaction Details


Details of experiments between O14745 and O15439
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF1
Fragment: 10-98
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.63
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.36
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NHERF1
Fragment: 146-237
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835