Interaction Details


Details of experiments between O14641 and Q13574
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DVL2
Fragment: 260-359
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.15
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.5
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DVL2
Fragment: 1-736
Construct: endogenous protein (Jurkat)

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: -0
Immobilized partner concentration (10-6M): 10
P value: 0.02
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 37942667
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))