Interaction Details


Details of experiments between O00592 and O43464
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PODXL
Fragment: 549-558
Site: PBM
Sequence: DLDEEEDTHL
Construct: biotin-ttds-DLDEEEDTHL

Protein B

Protein: HTRA2
Fragment: 355-458
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.52
PUBMED ID: 36115835