Interaction Details


Details of experiments between O00499 and P61978
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.53
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.84
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.43
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.67
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.43
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.67
PUBMED ID: N

Experiment series 4

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.43
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.66
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.43
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.66
PUBMED ID: N

Experiment series 6

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.4
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.6
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.4
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.6
PUBMED ID: N

Experiment series 8

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 1-463
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.26
Immobilized partner concentration (10-6M): 10
P value: 7.22
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.55
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 9

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.37
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.54
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.35
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.52
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.48
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.3
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.41
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.3
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.41
PUBMED ID: N

Experiment series 14

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.22
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.21
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.22
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.21
PUBMED ID: N

Experiment series 16

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.16
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.09
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.12
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.9
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.12
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.9
PUBMED ID: N

Experiment series 20

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.09
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 3.73
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 1-463
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.35
Immobilized partner concentration (10-6M): 10
P value: 5.77
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 22

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.88
PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 3.65
PUBMED ID: 38995680

Experiment series 24

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 3.57
PUBMED ID: 38995680

Experiment series 25

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 3.53
PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 27

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 29

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: -0
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 30

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 31

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 32

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 33

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: -0.07
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 34

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 35

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: -0
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 36

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.31
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.79
PUBMED ID: 38995680

Experiment series 37

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.59
PUBMED ID: 38995680

Experiment series 38

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.15
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.51
PUBMED ID: 38995680

Experiment series 39

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.47
PUBMED ID: 38995680

Experiment series 40

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.29
PUBMED ID: 38995680

Experiment series 41

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.08
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.19
PUBMED ID: 38995680

Experiment series 42

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.14
Holdup BI standard deviation: 0
Normalized holdup BI: 0.18
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.08
PUBMED ID: N

Experiment series 43

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.17
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.06
PUBMED ID: N

Experiment series 44

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 3.96
PUBMED ID: 38995680

Experiment series 45

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.14
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.95
PUBMED ID: N

Experiment series 46

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.11
Normalized holdup BI: 0.13
Normalized holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.94
PUBMED ID: N

Experiment series 47

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.08
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.11
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.82
PUBMED ID: N

Experiment series 48

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.09
Normalized holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.76
PUBMED ID: N

Experiment series 49

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.57
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.92
PUBMED ID: 38995680

Experiment series 50

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.4
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.6
PUBMED ID: 38995680

Experiment series 51

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.48
PUBMED ID: 38995680

Experiment series 52

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.3
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.4
PUBMED ID: 38995680

Experiment series 53

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.24
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.27
PUBMED ID: 38995680

Experiment series 54

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.22
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.21
PUBMED ID: N

Experiment series 55

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.21
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.18
PUBMED ID: 38995680

Experiment series 56

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.2
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.14
PUBMED ID: N

Experiment series 57

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.19
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.12
PUBMED ID: N

Experiment series 58

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 3.94
PUBMED ID: 38995680

Experiment series 59

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.93
PUBMED ID: N

Experiment series 60

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.8
PUBMED ID: N

Experiment series 61

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.61
PUBMED ID: N

Experiment series 62

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.34
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.77
PUBMED ID: 38995680

Experiment series 63

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.18
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.61
PUBMED ID: 38995680

Experiment series 64

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.59
PUBMED ID: 38995680

Experiment series 65

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.53
PUBMED ID: 38995680

Experiment series 66

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.12
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.36
PUBMED ID: 38995680

Experiment series 67

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.09
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.22
PUBMED ID: 38995680

Experiment series 68

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.19
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.13
PUBMED ID: N

Experiment series 69

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.09
PUBMED ID: N

Experiment series 70

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.07
PUBMED ID: N

Experiment series 71

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.81
PUBMED ID: N

Experiment series 72

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.78
PUBMED ID: N

Experiment series 73

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.08
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.69
PUBMED ID: N

Experiment series 74

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 6
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 75

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.53
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.85
PUBMED ID: 38995680

Experiment series 76

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.38
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.56
PUBMED ID: 38995680

Experiment series 77

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.38
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.56
PUBMED ID: N

Experiment series 78

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.37
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.55
PUBMED ID: 38995680

Experiment series 79

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.37
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.55
PUBMED ID: N

Experiment series 80

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.35
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.51
PUBMED ID: 38995680

Experiment series 81

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.35
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.51
PUBMED ID: N

Experiment series 82

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.33
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.47
PUBMED ID: 38995680

Experiment series 83

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.32
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.46
PUBMED ID: 38995680

Experiment series 84

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.31
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.42
PUBMED ID: 38995680

Experiment series 85

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.25
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.3
PUBMED ID: 38995680

Experiment series 86

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.25
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.3
PUBMED ID: N

Experiment series 87

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.19
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.13
PUBMED ID: 38995680

Experiment series 88

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.07
PUBMED ID: 38995680

Experiment series 89

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.07
PUBMED ID: N

Experiment series 90

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.15
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.01
PUBMED ID: 38995680

Experiment series 91

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.79
PUBMED ID: 38995680

Experiment series 92

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.79
PUBMED ID: N

Experiment series 93

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 3.53
PUBMED ID: 38995680

Experiment series 94

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.54
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.87
PUBMED ID: 38995680

Experiment series 95

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.35
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.51
PUBMED ID: 38995680

Experiment series 96

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.49
PUBMED ID: 38995680

Experiment series 97

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.33
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.47
PUBMED ID: 38995680

Experiment series 98

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.21
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.19
PUBMED ID: N

Experiment series 99

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.21
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.17
PUBMED ID: N

Experiment series 100

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 4.16
PUBMED ID: N

Experiment series 101

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.19
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.13
PUBMED ID: 38995680

Experiment series 102

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.06
PUBMED ID: 38995680

Experiment series 103

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.15
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.99
PUBMED ID: N

Experiment series 104

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
Measured pKd: 3.81
PUBMED ID: N

Experiment series 105

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 3.65
PUBMED ID: 38995680

Experiment series 106

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 107

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.42
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.81
PUBMED ID: 38995680

Experiment series 108

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.24
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 9
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.58
PUBMED ID: 38995680

Experiment series 109

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.21
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 9
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.52
PUBMED ID: 38995680

Experiment series 110

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 9
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.43
PUBMED ID: 38995680

Experiment series 111

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.1
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 9
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.12
PUBMED ID: 38995680

Experiment series 112

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.09
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 9
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 4.08
PUBMED ID: 38995680

Experiment series 113

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 9
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 114

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Bio E E Ado Ado-PRRGPPPPPPGRGGR-CONH2
Average holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.88
PUBMED ID: 38995680

Experiment series 115

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 284-298
Site: PxxP_3
Sequence: SPRRGPPPPPPGRGG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
Measured pKd: 3.52
PUBMED ID: 38995680

Experiment series 116

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 266-280
Site: PxxP_1
Sequence: PGRGGRPMPPSRRDY
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 117

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 283-297
Site: PxxP_2
Sequence: MSPRRGPPPPPPGRG
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 118

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 285-299
Site: PxxP_4
Sequence: PRRGPPPPPPGRGGR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 119

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 302-316
Site: PxxP_5
Sequence: SRARNLPLPPPPPPR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 120

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: HNRNPK
Fragment: 305-319
Site: PxxP_6
Sequence: RNLPLPPPPPPRGGD
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680