Interaction Details


Details of experiments between O00499 and O43426
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: 0.69
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.17
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1-1573
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 10
P value: 4.59
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.16
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: 0.34
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.49
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.33
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.48
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.06
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.85
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: BIN1
Fragment: 1-593
Construct: endogenous proteins (Jurkat)

Protein B

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2
Average holdup BI: -0.11
Immobilized partner concentration (10-6M): 10
P value: 0.26
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-12-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch5; Ncontrols = 12; Nsamples = 6; Npreys = 6006; threshold curvature: 1.54

Experiment series 8

Protein A

Protein: BIN1
Fragment: 1-593
Construct: endogenous proteins (Jurkat)

Protein B

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 10
P value: 0.08
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.72

Experiment series 9

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: 0.32
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.45
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.87
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.5
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 22

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 24

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.6
PUBMED ID: 38995680

Experiment series 25

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 27

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 29

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 30

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 31

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 32

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 33

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: -0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 34

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.15
PUBMED ID: 38995680

Experiment series 35

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.45
PUBMED ID: 38995680

Experiment series 36

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.39
PUBMED ID: 38995680

Experiment series 37

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.92
PUBMED ID: 38995680

Experiment series 38

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 39

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 40

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 41

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 42

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 43

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 44

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: 0.71
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.2
PUBMED ID: 38995680

Experiment series 45

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: 0.41
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.62
PUBMED ID: 38995680

Experiment series 46

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.36
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.53
PUBMED ID: 38995680

Experiment series 47

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: 0.25
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.29
PUBMED ID: 38995680

Experiment series 48

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.06
PUBMED ID: 38995680

Experiment series 49

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 50

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 51

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 52

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 53

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 54

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: 0.54
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.16
PUBMED ID: 38995680

Experiment series 55

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.37
PUBMED ID: 38995680

Experiment series 56

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.31
PUBMED ID: 38995680

Experiment series 57

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.89
PUBMED ID: 38995680

Experiment series 58

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.8
PUBMED ID: 38995680

Experiment series 59

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 60

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 61

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: -0.06
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 62

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 63

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: -0.08
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 64

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: 0.72
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.23
PUBMED ID: 38995680

Experiment series 65

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: 0.34
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.49
PUBMED ID: 38995680

Experiment series 66

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.31
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.43
PUBMED ID: 38995680

Experiment series 67

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.1
PUBMED ID: 38995680

Experiment series 68

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.91
PUBMED ID: 38995680

Experiment series 69

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 70

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 71

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 72

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 73

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 74

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: 0.7
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.18
PUBMED ID: 38995680

Experiment series 75

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: 0.37
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.55
PUBMED ID: 38995680

Experiment series 76

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.31
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.42
PUBMED ID: 38995680

Experiment series 77

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.17
PUBMED ID: 38995680

Experiment series 78

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.06
PUBMED ID: 38995680

Experiment series 79

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 80

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 81

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 82

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 83

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 84

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: 0.57
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.1
PUBMED ID: 38995680

Experiment series 85

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.23
PUBMED ID: 38995680

Experiment series 86

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.22
PUBMED ID: 38995680

Experiment series 87

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.93
PUBMED ID: 38995680

Experiment series 88

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.65
PUBMED ID: 38995680

Experiment series 89

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: -0.06
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 90

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 91

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 92

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 93

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: -0.08
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 94

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.17
PUBMED ID: 38995680

Experiment series 95

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.16
PUBMED ID: 38995680

Experiment series 96

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.53
PUBMED ID: 38995680

Experiment series 97

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.48
PUBMED ID: 38995680

Experiment series 98

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2
Average holdup BI: -0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 99

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 100

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 101

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 102

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 103

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680