Interaction Details

Details of experiments between O00499 and Q14289
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 1-1009
Construct: endogenous protein (Jurkat)

Average holdup BI: 0.74

Immobilized partner concentration (10^-6M): 10

P value: 3.73

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic binding equation

Date of measurement: 2022-06-29

Number of measurements: 3

Measured pK_d: 5.45

PUBMED ID: 38995680

Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: 0.27

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

Measured pK_d: 4.33

PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: 0.08

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

Measured pK_d: 3.71

PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0.07

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 3.61

PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: -0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: -0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: -0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: 0.1

Immobilized partner concentration (10^-6M): 8

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 4.15

PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: 0.04

Immobilized partner concentration (10^-6M): 8

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0.02

Immobilized partner concentration (10^-6M): 8

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: 0.29

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 4.39

PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: 0.08

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 3.67

PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: 0.14

Immobilized partner concentration (10^-6M): 10

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

Measured pK_d: 4.23

PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: 0.06

Immobilized partner concentration (10^-6M): 10

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

Measured pK_d: 3.78

PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0.02

Immobilized partner concentration (10^-6M): 10

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: 0.25

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

Measured pK_d: 4.3

PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: 0.09

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

Measured pK_d: 3.75

PUBMED ID: 38995680

Experiment series 22

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: 0.3

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

Measured pK_d: 4.41

PUBMED ID: 38995680

Experiment series 24

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: 0.09

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

Measured pK_d: 3.74

PUBMED ID: 38995680

Experiment series 25

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: 0.09

Immobilized partner concentration (10^-6M): 13

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

Measured pK_d: 3.91

PUBMED ID: 38995680

Experiment series 27

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: 0.05

Immobilized partner concentration (10^-6M): 13

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0.03

Immobilized partner concentration (10^-6M): 13

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-17

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 29

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 850-864
Site: PxxP_3
Sequence: LEFTGPPQKPPRLGA
Construct: Bio Ado Ado-LEFTGPPQKPPRLGA-CONH2

Average holdup BI: 0.52

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

Measured pK_d: 4.83

PUBMED ID: 38995680

Experiment series 30

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 708-722
Site: PxxP_1
Sequence: TAFQEPPPKPSRPKY
Construct: Bio Ado Ado-TAFQEPPPKPSRPKY-CONH2

Average holdup BI: 0.22

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

Measured pK_d: 4.21

PUBMED ID: 38995680

Experiment series 31

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PTK2B
Fragment: 834-848
Site: PxxP_2
Sequence: YMNDKSPLTPEKEVG
Construct: Bio Ado Ado-YMNDKSPLTPEKEVG-CONH2

Average holdup BI: 0.05

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-18

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680