Interaction Details


Details of experiments between O00499 and Q96P48
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 1-1450
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.74
Immobilized partner concentration (10-6M): 10
P value: 7.19
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.46
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.8
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.44
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.09
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.03
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.76
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.61
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.39
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.59
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: -0.23
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 22

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.62
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.41
PUBMED ID: 38995680

Experiment series 24

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.48
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.14
PUBMED ID: 38995680

Experiment series 25

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.13
PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 27

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 29

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 30

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.74
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.27
PUBMED ID: 38995680

Experiment series 31

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.68
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.14
PUBMED ID: 38995680

Experiment series 32

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.25
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.29
PUBMED ID: 38995680

Experiment series 33

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.08
PUBMED ID: 38995680

Experiment series 34

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.71
PUBMED ID: 38995680

Experiment series 35

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 36

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 37

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.66
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.41
PUBMED ID: 38995680

Experiment series 38

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.07
PUBMED ID: 38995680

Experiment series 39

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.85
PUBMED ID: 38995680

Experiment series 40

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 41

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 42

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 43

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 44

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.8
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.44
PUBMED ID: 38995680

Experiment series 45

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.68
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.13
PUBMED ID: 38995680

Experiment series 46

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.09
PUBMED ID: 38995680

Experiment series 47

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.75
PUBMED ID: 38995680

Experiment series 48

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 38995680

Experiment series 49

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.56
PUBMED ID: 38995680

Experiment series 50

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 51

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.77
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.34
PUBMED ID: 38995680

Experiment series 52

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.64
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.05
PUBMED ID: 38995680

Experiment series 53

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.08
PUBMED ID: 38995680

Experiment series 54

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.81
PUBMED ID: 38995680

Experiment series 55

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.67
PUBMED ID: 38995680

Experiment series 56

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.53
PUBMED ID: 38995680

Experiment series 57

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 58

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.72
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.42
PUBMED ID: 38995680

Experiment series 59

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.6
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.15
PUBMED ID: 38995680

Experiment series 60

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.84
PUBMED ID: 38995680

Experiment series 61

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.65
PUBMED ID: 38995680

Experiment series 62

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 63

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 64

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 65

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 109-123
Site: PxxP_1
Sequence: EGLPAAPPIPPRRSC
Construct: Bio Ado Ado-EGLPAAPPIPPRRSC-CONH2
Average holdup BI: 0.46
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.71
PUBMED ID: 38995680

Experiment series 66

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 110-124
Site: PxxP_2
Sequence: GLPAAPPIPPRRSCL
Construct: Bio Ado Ado-GLPAAPPIPPRRSCL-CONH2
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.03
PUBMED ID: 38995680

Experiment series 67

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 210-224
Site: PxxP_6
Sequence: PCPPEIPPKPVRLFP
Construct: Bio Ado Ado-PCPPEIPPKPVRLFP-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.68
PUBMED ID: 38995680

Experiment series 68

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 207-221
Site: PxxP_5
Sequence: SPPPCPPEIPPKPVR
Construct: Bio Ado Ado-SPPPCPPEIPPKPVR-CONH2
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.66
PUBMED ID: 38995680

Experiment series 69

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 136-150
Site: PxxP_3
Sequence: APDPVLPPLPAKRHL
Construct: Bio Ado Ado-APDPVLPPLPAKRHL-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 70

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 150-164
Site: PxxP_4
Sequence: LAELSVPPVPPRTGP
Construct: Bio Ado Ado-LAELSVPPVPPRTGP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 71

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ARAP1
Fragment: 233-247
Site: PxxP_7
Sequence: EVPEEGPGAPARVMT
Construct: Bio Ado Ado-EVPEEGPGAPARVMT-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680