Interaction Details


Details of experiments between O00499 and Q9ULL5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1-2036
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.58
Immobilized partner concentration (10-6M): 10
P value: 5.05
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.15
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.64
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.06
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.29
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.38
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.16
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.69
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.36
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.53
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 22

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 24

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 25

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 27

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 29

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 30

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 31

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 32

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 33

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 34

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 35

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.46
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.1
PUBMED ID: 38995680

Experiment series 36

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 38995680

Experiment series 37

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.13
PUBMED ID: 38995680

Experiment series 38

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.86
PUBMED ID: 38995680

Experiment series 39

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.03
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 40

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 41

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 42

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 43

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 44

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 45

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 46

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.69
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.15
PUBMED ID: 38995680

Experiment series 47

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.37
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.55
PUBMED ID: 38995680

Experiment series 48

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0.24
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.27
PUBMED ID: 38995680

Experiment series 49

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.75
PUBMED ID: 38995680

Experiment series 50

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.1
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 51

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 52

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 53

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 54

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 55

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 56

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 57

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.5
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.09
PUBMED ID: 38995680

Experiment series 58

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.37
PUBMED ID: 38995680

Experiment series 59

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.99
PUBMED ID: 38995680

Experiment series 60

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.78
PUBMED ID: 38995680

Experiment series 61

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.04
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 62

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 63

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 64

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 65

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 66

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 67

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 68

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.67
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.13
PUBMED ID: 38995680

Experiment series 69

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.36
PUBMED ID: 38995680

Experiment series 70

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.09
PUBMED ID: 38995680

Experiment series 71

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.68
PUBMED ID: 38995680

Experiment series 72

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.04
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 73

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 74

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 75

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 76

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 77

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 78

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 79

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.66
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.11
PUBMED ID: 38995680

Experiment series 80

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.32
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 38995680

Experiment series 81

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0.22
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.21
PUBMED ID: 38995680

Experiment series 82

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.75
PUBMED ID: 38995680

Experiment series 83

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 84

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 85

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 86

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 87

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 88

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 89

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 90

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.58
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.12
PUBMED ID: 38995680

Experiment series 91

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.21
PUBMED ID: 38995680

Experiment series 92

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.75
PUBMED ID: 38995680

Experiment series 93

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 38995680

Experiment series 94

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.04
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 95

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 96

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 97

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 98

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 99

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 100

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 101

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.09
PUBMED ID: 38995680

Experiment series 102

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.01
PUBMED ID: 38995680

Experiment series 103

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 104

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 105

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 106

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 107

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 108

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 109

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 110

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 111

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 112

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 113

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 114

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 115

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 116

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 117

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 118

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 119

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 120

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 121

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 122

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 123

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 124

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 125

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 126

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 127

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 128

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 129

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 130

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 131

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680