Interaction Details


Details of experiments between O00499 and Q8WXE0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1-1202
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 10
P value: 4.01
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.98
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: 0.57
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.92
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: 0.5
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.79
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: 0.49
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
Measured pKd: 4.77
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: 0.34
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.49
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.85
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.53
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: 0.3
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.42
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: 0.21
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
Measured pKd: 4.19
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.15
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 22

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 24

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 25

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: -0.02
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: -0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 27

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 29

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: 0.36
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.91
PUBMED ID: 38995680

Experiment series 30

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: 0.26
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.68
PUBMED ID: 38995680

Experiment series 31

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: 0.26
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
Measured pKd: 4.67
PUBMED ID: 38995680

Experiment series 32

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.3
PUBMED ID: 38995680

Experiment series 33

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 38995680

Experiment series 34

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 35

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 36

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 37

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 38

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: 0.53
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
Measured pKd: 4.84
PUBMED ID: 38995680

Experiment series 39

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: 0.51
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.82
PUBMED ID: 38995680

Experiment series 40

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: 0.44
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.69
PUBMED ID: 38995680

Experiment series 41

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: 0.42
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.65
PUBMED ID: 38995680

Experiment series 42

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.05
PUBMED ID: 38995680

Experiment series 43

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.59
PUBMED ID: 38995680

Experiment series 44

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.53
PUBMED ID: 38995680

Experiment series 45

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 46

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 47

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: 0.41
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.91
PUBMED ID: 38995680

Experiment series 48

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: 0.31
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 2
Measured pKd: 4.7
PUBMED ID: 38995680

Experiment series 49

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: 0.3
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.69
PUBMED ID: 38995680

Experiment series 50

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.24
PUBMED ID: 38995680

Experiment series 51

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.82
PUBMED ID: 38995680

Experiment series 52

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.77
PUBMED ID: 38995680

Experiment series 53

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 54

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 55

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 56

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: 0.57
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.92
PUBMED ID: 38995680

Experiment series 57

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: 0.49
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
Measured pKd: 4.78
PUBMED ID: 38995680

Experiment series 58

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.77
PUBMED ID: 38995680

Experiment series 59

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: 0.37
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.55
PUBMED ID: 38995680

Experiment series 60

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.84
PUBMED ID: 38995680

Experiment series 61

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.66
PUBMED ID: 38995680

Experiment series 62

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.48
PUBMED ID: 38995680

Experiment series 63

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 64

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 65

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: 0.58
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.94
PUBMED ID: 38995680

Experiment series 66

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: 0.51
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 2
Measured pKd: 4.8
PUBMED ID: 38995680

Experiment series 67

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: 0.51
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.8
PUBMED ID: 38995680

Experiment series 68

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: 0.37
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.56
PUBMED ID: 38995680

Experiment series 69

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.95
PUBMED ID: 38995680

Experiment series 70

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.58
PUBMED ID: 38995680

Experiment series 71

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 72

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 73

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 74

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: 0.5
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.95
PUBMED ID: 38995680

Experiment series 75

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: 0.42
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.81
PUBMED ID: 38995680

Experiment series 76

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: 0.42
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
Measured pKd: 4.81
PUBMED ID: 38995680

Experiment series 77

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.18
PUBMED ID: 38995680

Experiment series 78

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 79

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 80

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 81

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 82

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 83

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 785-799
Site: PxxP_4
Sequence: PATPPDPPRPKRRSH
Construct: Bio Ado Ado-PATPPDPPRPKRRSH-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.07
PUBMED ID: 38995680

Experiment series 84

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 862-876
Site: PxxP_5
Sequence: RARRKGPPPPPPKRL
Construct: Bio Ado Ado-RARRKGPPPPPPKRL-CONH2
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.03
PUBMED ID: 38995680

Experiment series 85

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 863-877
Site: PxxP_6
Sequence: ARRKGPPPPPPKRLS
Construct: Bio Ado Ado-ARRKGPPPPPPKRLS-CONH2
Average holdup BI: 0.05
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 2
Measured pKd: 3.48
PUBMED ID: 38995680

Experiment series 86

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 1121-1135
Site: PxxP_1
Sequence: PRLGPRPVPPPRPES
Construct: Bio Ado Ado-PRLGPRPVPPPRPES-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 87

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 683-697
Site: PxxP_2
Sequence: GGPEPLPLPPARSPS
Construct: Bio Ado Ado-GGPEPLPLPPARSPS-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 88

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 782-796
Site: PxxP_3
Sequence: AGPPATPPDPPRPKR
Construct: Bio Ado Ado-AGPPATPPDPPRPKR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 89

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 864-878
Site: PxxP_7
Sequence: RRKGPPPPPPKRLSS
Construct: Bio Ado Ado-RRKGPPPPPPKRLSS-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 90

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 960-974
Site: PxxP_8
Sequence: IKQRPKPAGPPPRET
Construct: Bio Ado Ado-IKQRPKPAGPPPRET-CONH2
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 91

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CASKIN2
Fragment: 969-983
Site: PxxP_9
Sequence: PPPRETPVPPGLDFN
Construct: Bio Ado Ado-PPPRETPVPPGLDFN-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680