Interaction Details


Details of experiments between O00499 and Q8IY57
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 1-180
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 10
P value: 5.72
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.15
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.49
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
Measured pKd: 4.78
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.04
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.01
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.26
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
Measured pKd: 4.68
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.51
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
Measured pKd: 4.81
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.3
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 2
Measured pKd: 4.68
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.51
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
Measured pKd: 4.81
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.5
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 2
Measured pKd: 4.8
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.33
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 2
Measured pKd: 4.62
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: YAF2
Fragment: 3-17
Site: PxxP_1
Sequence: DKKSPTRPKRQPKPS
Construct: Bio Ado Ado-DKKSPTRPKRQPKPS-CONH2
Average holdup BI: 0.41
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 2
Measured pKd: 4.62
PUBMED ID: 38995680