Interaction Details


Details of experiments between O00499 and Q12894
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 1-506
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.51
Immobilized partner concentration (10-6M): 10
P value: 3.23
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.01
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.1
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.57
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.84
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.17
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.08
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.08
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: 0.19
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.13
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.78
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: IFRD2
Fragment: 57-71
Site: PxxP_1
Sequence: RPPRPVPGMPRARKG
Construct: Bio Ado Ado-RPPRPVPGMPRARKG-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680