Interaction Details


Details of experiments between O00499 and P0DPB5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: POLR1D
Fragment: 1-122
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.58
Immobilized partner concentration (10-6M): 10
P value: 7.18
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.14
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))