Interaction Details

Details of experiments between O00233 and P59637
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PSMD9
Fragment: 105-223
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Average holdup BI: -0.1

Holdup BI standard deviation: 0.16

Immobilized partner concentration (10^-6M): 20

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835