Interaction Details


Details of experiments between O00192 and Q02410
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARVCF
Fragment: 953-962
Site: PBM
Sequence: AKPQPVDSWV
Construct: Biotin-Ahx-AKPQPVDSWV

Protein B

Protein: APBA1
Fragment: 743-822
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.34
Normalized holdup BI: 0.41
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.62
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ARVCF
Fragment: 953-962
Site: PBM
Sequence: AKPQPVDSWV
Construct: Biotin-Ahx-AKPQPVDSWV

Protein B

Protein: APBA1
Fragment: 655-741
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835