Interaction Details


Details of experiments between O00151 and O95049
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDLIM1
Fragment: 1-87
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TJP3
Fragment: 910-919
Site: PBM
Sequence: GYDWGPATDL
Construct: Biotin-Ahx-GYDWGPATDL
Average holdup BI: 0.3
Normalized holdup BI: 0.3
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.41
PUBMED ID: 36115835