Interaction Details


Details of experiments between NC_000001.11 and Q9P016
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: 51_HAP
Fragment: 203681832-203681862
Construct: Biotin-GAATTGAGAGGTATCTTACCGCTCCCACTCC

Protein B

Protein: THYN1
Fragment: 1-225
Construct: endogenous protein (K562)
Average holdup BI: 0.96
Immobilized partner concentration (10-6M): 5
P value: 4.9
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 6.68
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: 4945_HAP
Fragment: 203681644-203681669
Construct: Biotin-TCAGCCCTCCGTTTTGTCACCTACAC

Protein B

Protein: THYN1
Fragment: 1-225
Construct: endogenous protein (K562)
Average holdup BI: 0.89
Immobilized partner concentration (10-6M): 5
P value: 9.86
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 6.19
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 3

Protein A

Protein: 4945_WT
Fragment: 203681644-203681669
Modification: rs10751449/rs10736845
Construct: Biotin-TCAGCCCTCCGTATCGTCACCTACAC

Protein B

Protein: THYN1
Fragment: 1-225
Construct: endogenous protein (K562)
Average holdup BI: 0.93
Immobilized partner concentration (10-6M): 5
P value: 10.84
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 6.42
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 4

Protein A

Protein: 51_WT
Fragment: 203681832-203681862
Modification: rs10751451
Construct: Biotin-GAATTGAGAGGTATCTTATCGCTCCCACTCC

Protein B

Protein: THYN1
Fragment: 1-225
Construct: endogenous protein (K562)
Average holdup BI: 0.97
Immobilized partner concentration (10-6M): 5
P value: 7.53
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 6.77
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))