Interaction Details


Details of experiments between NC_000001.11 and Q9BVC5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: 4945_HAP
Fragment: 203681644-203681669
Construct: Biotin-TCAGCCCTCCGTTTTGTCACCTACAC

Protein B

Protein: C2orf49
Fragment: 1-232
Construct: endogenous protein (K562)
Average holdup BI: 0.7
Immobilized partner concentration (10-6M): 5
P value: 8.46
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.66
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: 51_HAP
Fragment: 203681832-203681862
Construct: Biotin-GAATTGAGAGGTATCTTACCGCTCCCACTCC

Protein B

Protein: C2orf49
Fragment: 1-232
Construct: endogenous protein (K562)
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 5
P value: 0.43
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 3

Protein A

Protein: 4945_WT
Fragment: 203681644-203681669
Modification: rs10751449/rs10736845
Construct: Biotin-TCAGCCCTCCGTATCGTCACCTACAC

Protein B

Protein: C2orf49
Fragment: 1-232
Construct: endogenous protein (K562)
Average holdup BI: 0.67
Immobilized partner concentration (10-6M): 5
P value: 6.05
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.62
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 4

Protein A

Protein: 51_WT
Fragment: 203681832-203681862
Modification: rs10751451
Construct: Biotin-GAATTGAGAGGTATCTTATCGCTCCCACTCC

Protein B

Protein: C2orf49
Fragment: 1-232
Construct: endogenous protein (K562)
Average holdup BI: -0.07
Immobilized partner concentration (10-6M): 5
P value: 1.92
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))