Interaction Details


Details of experiments between K9N5R3 and Q9H190
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV

Protein B

Protein: SDCBP2
Fragment: 103-192
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 3.76
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV

Protein B

Protein: SDCBP2
Fragment: 187-271
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.31
Holdup BI standard deviation: 0.36
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835