Interaction Details

Details of experiments between K9N5R3 and Q9BYB0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV

Protein B

Protein: SHANK3
Fragment: 562-670
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.29

Holdup BI standard deviation: 0.23

Immobilized partner concentration (10^-6M): 20

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835